![]() Please refer to the loading limit per shelf indicated for your incubator or contact your sales representative for more information. *Some incubators and environmental chambers will require reinforced shelving to support the weight of a shaker. Extreme Environment Shakers are designed for use in. Thermo Scientific Forma 3940 Series Environmental Chambersĩ0kg fixed reinforced shelves* ( 1900005) High Performance Orbital Shaker Designed to Withstand Extreme Environments Up To 100 Humidity. Heracell VIOS 250i CO 2incubator-Stainless Heracell VIOS 160i CO 2incubator-Stainless In addition, the performance of the shaker inside the CO 2 incubator should be tested to ensure it doesn´t lead to an increased contamination risk by condensation.Forma Series II 3110 Water-Jacketed CO 2 Incubatorĩ0kg fixed reinforced shelves * ( 1900005) To ensure the exclusion of moisture inside the shaker, electronics and drive-related mechanical parts should be sealed. Therefore, the shaker inside the CO 2 incubator should facilitate the detection and fast, thorough removal of medium spills. In addition to the use of aseptic techniques, it is worth thinking about the cleanability of a shaker as a structural defense, especially given the labor invested in cleaning over it´s lifetime.Ĭontaminants preferably grow on moist surfaces or in liquids. Regardless of its type, contamination prevention is crucial to avoid additional expenses by loss of sample material, the need to repeat experiments, combatting the contamination itself, and possible downtimes imposed by these measures. It offers a large shaking capacity with a small footprint and many features allowing straightforward, safe handling of any culture or product. The ISF1-Z complies with GMP (good manufacturing practice) requirements and regulations. In addition, cross-contaminating eukaryotic cell lines that can be introduced by earlier or simultaneous projects inside the same device. The ISF1-Z incubator shaker is suitable for applications in laboratories, research centers and production facilities. ![]() The warm, humid atmosphere inside the CO 2 incubator and nutrient-rich media used for mammalian cell cultivation provide ideal growth conditions for contaminants. Shaker impact on contamination prevention In this article, we focus on the challenges of putting a shaker into a CO 2 incubator. Two systems are commonly used: a) CO 2 resistant shakers that are placed inside a traditional CO 2 incubator and b) CO 2 incubator with an integrated shaker (Read more about the advantages and disadvantages of both systems and proper selection in our White Paper ). Appropriate growth conditions are provided by a CO 2-enriched atmosphere with high relative humidity that requires specialized devices. This is especially critical because shake flask agitation increases medium evaporation. Furthermore, the volume of the medium needs to be maintained to avoid harmful impacts by significant shifts in concentration of metabolites, salts, etc. It combines precise temperature and CO control with an excellent laboratory shaker. To ensure optimized cell growth, the temperature and pH of the medium needs to be controlled. The S41i CO incubator is specifically designed for non-adherent cells. for the expression of recombinant proteins or the production of bioreactor starter-cultures (inoculum). Mammalian cells in suspension are mainly cultivated in shake flasks to improve aeration and the exchange of nutrients and metabolic waste products in the cell-surrounding medium. Introduction - Why putting a shaker into a CO 2 incubator? This CO2 shaking incubator has the stainless steel frames with heating function around door, it can adjust the temperature as users need.
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